Figure 2.

DT-A selectively ablates cholinergic neurons in lesioned striatum of ChAT-Cre mice. A, Despite elimination of ChAT immunolabeling (green), expression of the general neuronal marker, NeuN (red), is similar in lesioned striatum of mice injected with AAV-DT-A compared with AAV-EYFP. The dotted white line and arrow indicate the injection needle track. B, Immunolabeling for DARPP-32 (dopamine- and cAMP-regulated phosphoprotein Mr 32 kDa), a marker of striatal medium spiny neurons, is also unaffected by AAV-DT-A. ac, Anterior commissure. Scale bar, 40 μm.