Table 1. Summary of experimental conditions.
Experimenta | Site | Purpose | Light (µmol photons m−2 s−1) | Temperature (°) | Feeding schedule |
---|---|---|---|---|---|
1, Apo | Stanford | Gene expressionb | 25 (12L:12D) | 27 | Every 2 d |
1, Sym | Stanford | Transcriptome assembly and gene expressionc | 25 (12L:12D) | 27 | Every 2 d |
2, Apo | Cornell | Gene expressiond | 0 | 25 | Unfed 2 wk |
2, Sym | Cornell | Transcriptome assembly and gene expressione | 18–22 (12L:12D) | 25 | Unfed 2 wk |
3, Apo | Cornell | RT-qPCR | 18–22 (12L:12D) | 25 | Every 2 d |
3, Sym | Cornell | RT-qPCR | 18–22 (12L:12D) | 25 | Every 2 d |
Apo, aposymbiotic anemones; Sym, symbiotic anemones.
Approximately 49 million 36-bp single-end reads (accession number SRR612167).
Approximately 200 million 101-bp paired-end reads (accession number SRR610288) and 51 million 36-bp single-end reads (accession number SRR612166).
Approximately 80 million 101-bp paired-end reads (accession number SRR612165).
Approximately 83 million 101-bp paired-end reads (accession number SRR696732).