Table 2. Biochemical Analysis of Whole Spiroplasma Cells.
Analyte | Weight(µg) | SD(µg) | Percent | MethodsReference |
Total protein 1 | 728.94 | 2.11 | 71.3 | 11.c. Protein |
Unconjugatedprotein2 | 226.91 | 5.88 | 22.2 | footnote 2 |
Lipoprotein | 285.35 | 6.31 | 27.9 | 11.d. Protein |
Glycoprotein | 216.50 | 3.99 | 21.2 | 11.d. Protein |
Total lipid 1 | 80.46 | 0.65 | 7.9 | 11.c. Lipid |
Free lipid3 | 29.72 | 1.09 | 2.9 | footnote 3 |
Fatty acid | 16.92 | 0.82 | 1.7 | 11.d. Lipid |
Phospholipid | 3.66 | 0.37 | 0.4 | 11.d. Lipid |
Cholesterol | 22.84 | 1.01 | 2.2 | 11.d. Lipid |
Glycolipids | 7.31 | 0.26 | 0.7 | 11.d. Lipid |
Total carbohydrate 1 | 82.61 | 0.49 | 8.1 | 11.c. Carbohydrate |
Free carbohydrate4 | 12.98 | 0.99 | 1.3 | 11.d. Carbohydrate |
Total DNA | 41.06 | 0.06 | 4.0 | 11.c. Nucleic acid s |
Total RNA | 88.21 | 0.85 | 8.6 | 11.c. Nucleic acids. |
Total dry mass of analyzed cells was 1,022 µg. Individual values are given as absolute weights (in µg) within this total amount, as well as in percent of the total dry mass. n = 3 analyses per sample.
Total protein, lipid, and carbohydrate content are the sum of all subtypes.
Unconjugated protein is defined as pure protein or peptide free of detectable carbohydrate or lipid; it is derived by subtracting the sum of lipoprotein and glycoprotein from total protein.
Free lipid is defined as lipids with no detectable carbohydrate or protein; it is derived by subtracting the sum of lipid subtypes from total lipid.
Free carbohydrate is defined as carbohydrates with no detectable lipid or protein.