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. 2014 Feb 21;9(2):e87921. doi: 10.1371/journal.pone.0087921

Table 2. Biochemical Analysis of Whole Spiroplasma Cells.

Analyte Weight(µg) SD(µg) Percent MethodsReference
Total protein 1 728.94 2.11 71.3 11.c. Protein
Unconjugatedprotein2 226.91 5.88 22.2 footnote 2
Lipoprotein 285.35 6.31 27.9 11.d. Protein
Glycoprotein 216.50 3.99 21.2 11.d. Protein
Total lipid 1 80.46 0.65 7.9 11.c. Lipid
Free lipid3 29.72 1.09 2.9 footnote 3
Fatty acid 16.92 0.82 1.7 11.d. Lipid
Phospholipid 3.66 0.37 0.4 11.d. Lipid
Cholesterol 22.84 1.01 2.2 11.d. Lipid
Glycolipids 7.31 0.26 0.7 11.d. Lipid
Total carbohydrate 1 82.61 0.49 8.1 11.c. Carbohydrate
Free carbohydrate4 12.98 0.99 1.3 11.d. Carbohydrate
Total DNA 41.06 0.06 4.0 11.c. Nucleic acid s
Total RNA 88.21 0.85 8.6 11.c. Nucleic acids.

Total dry mass of analyzed cells was 1,022 µg. Individual values are given as absolute weights (in µg) within this total amount, as well as in percent of the total dry mass. n = 3 analyses per sample.

1

Total protein, lipid, and carbohydrate content are the sum of all subtypes.

2

Unconjugated protein is defined as pure protein or peptide free of detectable carbohydrate or lipid; it is derived by subtracting the sum of lipoprotein and glycoprotein from total protein.

3

Free lipid is defined as lipids with no detectable carbohydrate or protein; it is derived by subtracting the sum of lipid subtypes from total lipid.

4

Free carbohydrate is defined as carbohydrates with no detectable lipid or protein.