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. 2014 Feb 21;9(2):e88865. doi: 10.1371/journal.pone.0088865

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© 2014 Mallya et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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m⁷-GTP pull down assays of VAL and OCI-LY1 cells treated with 100 nM MLN0128, 500 nM PP242 or 10 nM rapamycin for 4 hours. Proteins from the cap bound fraction were detected by western blotting along with the total cellular proteins. Results are representative of several independent experiments.