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. 2014 Feb 21;9(2):e88865. doi: 10.1371/journal.pone.0088865

Figure 6. Knockdown of eIF4E increases VAL cell sensitivity to MLN0128.

Figure 6

VAL and OCI-LY1 cells stably expressing eIF4E shRNA or scrambled shRNA control were treated for 48 hours with 100 nM MLN0128. Cell death was measured by Annexin V and PI staining. Results represent the percentage of cells that are Annexin V positive only and are averaged for 3 different experiments. Statistical significance was measured using a student’s t-test (paired, two-tailed) with error bars representing SEM (*p<0.05, **p<0.01 ***p<0.001,****p<0.0001). Shown below the graphs are western blots depicting the efficiency of eIF4E knockdown compared to the scrambled shRNA controls.