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. 2014 Feb 21;9(2):e88894. doi: 10.1371/journal.pone.0088894

Figure 2. KLK1 mediated AGE-BSA induced IL-8 and ICAM-1 expression in PTEC.

Figure 2

Cells were incubated with 0.1 or 0.5/ml AGE-BSA for 6 h, and gene expression was determined by real-time PCR analysis. AGE induced KLK1 mRNA expression in PTEC (A). Cells were transfected with KLK1-specific siRNA, and the endogenous protein level was determined by Western blot analysis (B). Transfected cells were incubated with 0.5 mg/ml AGE for 48 h, and protein expression of cytokine was detected in culture medium by ELISA. AGE -induced IL-8 (C) and ICAM-1 (D) protein expression was inhibited by KLK1 gene silencing. ***p<0.001 compared with control, δP<0.05 compared with mock transfection and #p<0.05; ##p<0.01 compared with mock transfection incubated with AGE.