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β1-adrenoceptors phosphorylation above basal level after 15-minute application of 10 µM isoproterenol or 10 µM isoproterenol+PKA inhibitor H-89. Experimental data from Freedman et al. [32] are shown with black bars with errors, simulation data are shown with gray bars. Effect of H-89 was simulated by setting [PKA]tot = 0 µM.
