. 2014 Feb 21;9(2):e89027. doi: 10.1371/journal.pone.0089027

Table 2. SAXS data collection and statistics.

	PerR	PerR-DNA mixture
		1∶1	2∶1	4∶1
Data-collection parameters
Instrument	Synchrotron 23A SWAXS endstation of NSRRC
Beam geometry	0.5 mm dia. beam
Wavelength (Å)	0.8857	0.82825	0.82825	0.82825
q range (Å⁻¹)	0.0085–0.37	0.007–0.39	0.007–0.39	0.007–0.39
Exposure time (s)	300^a	300^a	300^a	300^a
Concentration range (µM)	20.0	Protein 26.5	Protein 26.5	Protein 26.5
	68.1	DNA 26.5	DNA 13.3	DNA 6.63
	177.6
Temperature (K)	288	288	288	288
Structural parameters
R_g (Å) [from P(r)]	24.58±0.04	39.11±0.04	38.62±0.06	36.59±0.08
R_g (Å) [from Guinier]	24.8±0.4	38.9±0.3	38.1±0.1	35.5±0.2
I(0) (cm⁻¹) [from Guinier]	0.033	0.12	0.08	0.05
	0.113
	0.352
D _max (Å)	∼77	∼125	∼125	∼125
Porod volume estimate (Å³)	∼58100^b	∼354000	∼335000	∼122000
Dry volume calculated from sequence (Å³)	37682	N/A	N/A	N/A
Molecular-mass determination
Partial specific volume (cm³g⁻¹)	0.73	N/A	N/A	N/A
Contrast (Δρ*10⁻¹⁰cm⁻²)	3.148	N/A	N/A	N/A
Molecular mass Mr	∼35	∼80	∼50	∼40
[estimated from Porod Volume] kDa
Calculated monomeric M_r from sequence	37700	N/A	N/A	N/A
Software employed
Primary data reduction	NSRRC 23A SWAXS package
Data processing	SCATTER 1.7 and ATSAS 2.4
Ab initio analysis	DAMMIN	N/A	N/A	N/A
Validation and averaging	DAMAVER	N/A	N/A	N/A
Rigid-body modelling	N/A	N/A	N/A	N/A
Computation of model intensities	FoXS	FoXS	FoXS	FoXS
Envelope representations	Chimera	N/A	N/A	N/A
Minimal ensemble analysis	N/A	MES	MES	MES

a. 300s with 60s single exposure time for 5 successive exposures.

b. Only globular proteins with smooth surfaces: “Porod volume” ∼ “Dry volume”; uncertainty is contributed mainly from Q-invariant (upper limit of 20% is used with NSRRC 23A SWAXS)”.