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. 2014 Feb 21;9(2):e89390. doi: 10.1371/journal.pone.0089390

Figure 2. Effect of Tax on BRCA1 activation by E2 and 53PB1.

Figure 2

(A) MCF-7 cells were co-transfected with 1.5 µg of BARCA1-Luc reporter and the plasmids expressing 53PB1 and/or Tax. Where indicated, E2 (20 nM) was added to the cultures 5 hr before harvesting the cells for analyzing the reporter expression. (B) and (C) MCF-7 cells were co-transfected with 1.5 µg of BARCA1-Luc reporter and the plasmids expressing different Tax varients without (B) or with (C) E2 treatment. As above, E2 was added 5h before harvesting the cells for Luciferase activity measurement in the cell lysates at 24 h post-transfection. (D) MCF-7 cells were co-transfected with 1.5 µg of the plasmids expressing different Tax variants with E2 treatment and at 24h post transfection the BRCA1 mRNA levels were examined as detailed in “Material and Methods” section. The presented results are an average of three repeated experiments ± SE.