Figure 2. Quercetin attenuated H₂O₂-induced apoptosis in blastocysts.

(A) Representative images of TUNEL positive cells in mouse blastocysts after treatment with H₂O₂ or H₂O₂ plus Que during the zygote stage. TUNEL positive cells were stained with Alexa Fluor 488 dye and all nuclei were labeled with Hoechst 33342 (blue). Bar, 50 μm. (B) Quantification of the total cell numbers per blastocyst. (C) Percentage of TUNEL positive cells per blastocyst. Data are presented as mean ± SEM from three independent experiments. N shows the number of blastocysts. ^* P<0.05, ^** P<0.01, ^*** P<0.001 vs. the control group; ^# P<0.05, ^## P<0.01, ^### P<0.001 vs. the H₂O₂-treated group (One-way ANOVA).