Figure 6. Fluorescent images of epithelial cells and tracheal branches.

(a) Low-magnification image of compartments CuA₁ and M₃ stained with MitoTracker Orange for mitochondria (in red) and SYBR Green-1 for nuclei (in green), immediately after pupation. The red arrowhead indicates the organizing center for the border symmetry system, which is resistant to staining. This non-stained black area was not detected in the adjacent compartment where no eyespot exists in adult wings. Also see Figure 5a. The boxed region is magnified in (b). (b) High-magnification image of the epithelial cells boxed in (a). Yellow dots indicate mitochondrial DNA. (c) Optical cross section of the stained epithelial cells. The diameter of a single cell is indicated by a double-headed arrow. Cellular nucleus is encircled by red dots. Note the mitochondrial distribution on the dorsal surface of the cell. Yellow regions indicate mitochondrial DNA. (d, e) Compartment CuA₁ stained with DiBAC₄(3). Tracheae are stained well, and the epithelial cells are weakly stained. A portion of a tracheal knob is indicated by red arrowheads to demonstrate its vigorous movement.