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. 2014 Feb 21;9(2):e89631. doi: 10.1371/journal.pone.0089631

Figure 3. Application of targeted fibroblasts as nuclear donors in SCNT.

Figure 3

(A) PCR results demonstrating the presence of the targeted modification in SCNT-generated blastocysts from targeted 3142 fibroblasts. The control blastocyst was generated by SCNT using non-transfected 3142 fibroblasts (WT). One blastocyst was not tested (NT) due to technical issues. (B) Images of the two bovine SCNT-derived blastocysts are shown.