Table 2. Comparison of value ranges describing particular intronic parameters in the test set of G⁺¹ mutations.

	Splicing-affecting mutations(N = 13)			Non-affecting mutations(N = 12)			M-W test
	median	min	max	median	min	max	P
PPS length	8	4	18	14	6	17	0.026
Py in 25 bp	16	11	20	19	13	21	0.034
Py in 50 bp	29	21	37	34	26	38	0.053
BS score (Kol)	2.95	2.50	3.55	3.05	2.60	3.85	0.431
BS perc. (Kol)	0.25	0.04	0.84	0.36	0.07	0.98	0.431
BS to 3′ss dist. (Kol)	24	20	34	34	25	41	0.009
BS to PPT dist. (Kol)	0	0	6	0	0	5	0.844
BS score (Schwartz)	3	2	4	2.5	2	5	0.847
BS perc. (Schwartz)	0.05	0.00	0.32	0.19	0.00	0.32	0.847
BS to 3′ss dist. (Schwartz)	45	8	107	28.5	19	95	0.512
BS to PPT dist. (Schwartz)	24	2	88	14	−5	72	0.335
PPT length (Kol)	22	17	33	29	22	40	0.010
PPT to 3′ss dist. (Kol)	2	1	5	2	1	6	0.844
PPT score (Kol)	0.8	0.68	0.95	0.85	0.69	0.91	0.491
PPT perc. (Kol)	0.56	0.10	0.98	0.69	0.13	0.90	0.431
PPT length (Schwartz)	12.5	6	39	15.5	6	35	0.149
PPT to 3′ss dist. (Schwartz)	3.5	2	25	4	2	7	0.729
PPT score (Schwartz)	10.50	5.44	21.56	14.22	6.00	24.03	0.126
PPT perc. (Schwartz)	0.57	0.07	0.98	0.82	0.11	0.99	0.119

The BS and PPT parameters were predicted using prediction programs provided by Kol et al. [10] or Schwartz et al. [11] using the Sroogle engine. Computational predictions and other sequence parameters for an experimentally confirmed test set of splicing-affecting and non-affecting G⁺¹ mutations were subjected to statistical analysis using the Mann-Whitney test (see Table S1 File S1 for details). Significant differences (p<0.05) are marked in bold. dist. = distance, BS = branch point site, M-W test = Mann-Whitney test, perc. = percentile, PPS = the longest uninterrupted polypyrimidine stretch Py = number of pyrimidines (in 25 or 50 nt upstream from 3′ss).