Skip to main content
. 2014 Feb 21;9(2):e89743. doi: 10.1371/journal.pone.0089743

Figure 2. Destabilization of the cortical cytoskeleton enhances microvesicle release by SLO-damaged cells and potentiates their survival.

Figure 2

(A) Enhanced release of microvesicles by SLO-damaged cells, which were treated with latrunculin A. (B) Latrunculin A protects HEK 293 cells from SLO-induced cell lysis. (C) Diminished release of microvesicles by SLO-damaged cells which were treated with jasplakinolide. (D) Treatment with jasplakinolide results in increased cell lysis. (E) Calpeptin reduces microvesicle release by SLO-damaged cells. (F) SLO induces an increased rate of lysis in calpeptin-treated cells. **p<0.001, *p<0.01. (G) Amounts of annexin A1 shed within microvesicles were analyzed by Western Blotting in culture supernatants of SLO-treated cells pre-treated with either latrunculin (Latr/SLO), jasplakinolide (Jasp/SLO), cells treated with latrunculin without SLO treatment (Latr) or cells treated with DTT only (Contr). **p<0.001.