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Eugenol-dependent apoptosis is mediated through down-regulation of E2F1 and survivin. (A) Total RNA and proteins were extracted from MDA-MB-231 cells expressing either control-shRNA or E2F1-shRNA and used for RT-PCR and western blot analysis. (B) MDA-MB-231 cells were treated as shown for 72 hrs, and then apoptosis was assessed by annexinV/PI like in Figure 1B. Data are presented as means ± S.D.
