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. 2013 Jan 17;40(2):351–361. doi: 10.1093/schbul/sbs195

Fig. 1.

Fig. 1.

Gene and protein expression profiling of presynaptic GABAergic markers in prefrontal cortex following prenatal immune activation. (a) The graph depicts the 2(-Delta C(T)) values for each individual control (CON; white symbols) or poly(I:C)-exposed (POL, gray symbols) offspring at peripubertal (P) or adult (A) age. Individual mRNA levels are given for the 2 isoforms of the rate limiting enzyme for GABA biosynthesis (GAD65 and GAD67) and the vesicular GABA transporter (VGAT). The bars represent the mean of individual 2(-Delta C(T)) values for each group/age. *P < .05, **P < .01, and ***P < .001, based on independent Student’s t tests (two-tailed). (b) GAD65/67 protein levels for individual POL and CON offspring at peripubertal (P) or adult (A) age assessed using Western blot analysis. The photomicrographs show Western blot samples for GAD65/67 protein in the prefrontal cortex of 3 representative CON and POL offspring. β-actin is shown as control for comparison. *P < .05, based on independent Student’s t tests (two-tailed). (c) VGAT protein levels for individual POL and CON offspring at peripubertal (P) or adult (A) age assessed using Western blot analysis. The photomicrographs depict Western blot samples for VGAT protein in the prefrontal cortex of 3 representative CON and POL offspring. β-actin is shown as control for comparison. *P < .05, based on independent Student’s t tests (two-tailed). N(CON/peripuberty) = 9, N(POL/ peripuberty) = 7, N(CON/adulthood) = 11, N(POL/ adulthood) = 9.