Table 1.

Sequences of Forward and Reverse Primers Used in Real-Time Polymerase Chain Reaction Analysis

Gene	Forward Primer	Reverse Primer	Probe
gad65	5’-ACCGAGAAGGCTATGAAATGG-3’	5’-TCTGGCTTTAATCACTGGCG-3’	5’-AGGCGGCTCATTCTCTCTTCATTGTC-3’
gad67	5’-GCAGATCCTGGTTGACTGTAG-3’	5’-GAACATATTGGTATTGGCAGTCG-3’	5’-TGGTTTAGCTGGTGAATGGCTGACA-3’
vgat	5’-ACGACAAACCCAAGATCACG-3’	5’-AAGATGATGAGGAACAACCCC-3’	5’-CAGCACGAACATGCCCTGAATGG-3’
alpha1	5’-TGAGAGCTGAATGCCCAATG-3’	5’-TCTGCTACAACCACTGAACG-3’	5’-CCTGCCCACTAAAATTCGGAAGCTATGC-3’
alpha 2	5’-CCATGAGGCTTACAGTCCAAG-3’	5’-ACGGAGTCAGAAGCATTGTAAG-3’	5’-CGTAGCTTCCAAATTTCAGTGGGCA-3’
alpha 3	5’-ACAATATGACCACACCCAACA-3’	5’-AGCTTCCAAACTTCAGTGGG-3’	5’-CAATACACGCTGAATGCCCCATGC-3’
alpha 4	5’-GCCTGCCCTTTGAAATTTGG-3’	5’-GATACAGTCTGCCCAATGAGG-3’	5’-ATCTACACCTGGACCAAAGGCCC-3’
alpha 5	5’-GGGAATGGACAATGGAATGC-3’	5’-TGTCATTGGTCTCGTCTTGTAC-3’	5’-CATTTGCGAAAAGCCAAAGTGACTGGA-3’
nkcc1	5’-GCATTCAATCCGTCTTTCTGG-3’	5’-GGCCACAGATCATTAAACCAAC-3’	5’-AGTAAAGCAGGCCGTGAGTTTGGA-3’
kcc2	5’-TCAAACAGATGCACCTCACC-3’	5’-CCTCTGGCTTCTCCTCATTG-3’	5’-CTCCCGTTCCCGCTCGTTCTT-3’
36B4	5’-AGATGCAGCAGATCCGCAT-3’	5’-GTTCTTGCCCATCAGCACC-3’	5’-CGCTCCGAGGGAAGGCCG-3’
β-actin	5’-ACCTTCTACAATGAGCTGCG-3’	5’-CTGGATGGCTACGTACATGG-3’	5’-TCTGGGTCATCTTTTCACGGTTGGC-3’
18S rRNA	5’-GTAACCCGTTGAACCCCATT-3’	5’-CCATCCAATCGGTAGTAGCG-3’	5’-TGCAATTATTCCCCATGAACGAGG-3’