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. Author manuscript; available in PMC: 2014 Dec 20.
Published in final edited form as: ACS Synth Biol. 2013 Aug 22;2(12):724–733. doi: 10.1021/sb400076r

Figure 4. Chimeric capsid intactness determined by nuclease sensitivity and TEM imaging.

Figure 4

(a) Q-PCR was used to determine the percentage of viral genomes remaining after treatment with benzonase. Percentage protected represents benzonase treated samples normalized to a sham condition lacking nuclease. Chimeras are sorted horizontally based on their genomic titers compared to wt capsids; diamonds at the top represent relative titers of each chimera before nuclease treatment compared to parental capsids as in Fig. 3. Error bars indicate the standard error of the mean (SEM) calculated from three independent experiments. Transmission electron microscopy of (b) AAV2, (c) AAV4, and (d) chimera 438–633 show that AAV2 and AAV4 capsids exclude negative stain whereas the chimeric 438–633 capsids contain negative stain (dark centers). Images were taken at 40,000x magnification; insets were taken at 150,000x magnification.