Figure 4. Role of NF-κB on AT₁R expression in GRK4γ WT- and hGRK4γ 142V-transduced A10 cells.

A. EMSA of nuclear protein from A10 cells. Binding activity of AT₁R gene promoter (-350 bp and -363 bp), containing an NF-κB site, was examined in nuclear proteins from hGRK4γ WT- (lane 2) and hGRK4γ 142V (lane 3)-transduced A10 cells by EMSA. No nuclear extracts (lane 1) or fifty times unlabeled probe (lane 4) were added to the reaction mixture and served as negative controls. B. Effect of NF-κB on GRK4-mediated regulation of AT₁R protein expression in A10 cells. hGRK4γ WT- or hGRK4γ 142V-transduced cells were treated with or without the NF-κB inhibitor BAY11-7082 (20 μM) for 24hr. Results are expressed as the ratio of AT₁R and α-actin (n = 5, *P<0.05 vs. vehicle).