Figure 5. Colocalization and co-immunoprecipitation of GRK4 and AT₁R in hGRK4γ WT- and hGRK4γ 142V-transduced A10 cells.

A: Colocalization of GRK4 and AT₁R in hGRK4γ WT- and hGRK4γ 142V-transduced A10 cells. The cells were washed, fixed, and immunostained for GRK4 and AT₁R, as described in the Methods. Colocalization appears as purple after merging the images of AMCA-tagged GRK4 (blue) and rhodamine-tagged AT₁R (red). B: Co-immunoprecipitation of GRK4 and AT₁R in hGRK4γ WT- and hGRK4γ 142V-transduced A10 cells. The cells were immunoprecipitated with GRK4 antibodies and immunoblotted with AT₁R antibodies (*P<0.05 vs. control, n=4, ANOVA, Holm-Sidak test). One immunoblot (43 kDa) is depicted in the inset: (PC = positive control, WT = hGRK4γ WT-transduced A10 cells, 142V = hGRK4γ 142V-transduced A10 cells, NC = negative control. For the positive control, AT₁R antibody was used and for the negative control, IgG was used instead of GRK4 antibody as the immunoprecipitants.