Fig. 3.

Neuralisation of iPSCs was differentiated at high density in monolayer culture in the presence of SMAD inhibitors. a Uniform PAX6 staining of early neuroepithelial cells. b Rosette formation by neural progenitors after passaging of neuroepithelial cells. The apical lumen of the rosette is stained with the tight junction protein ZO-1, whilst acetylated tubulin forms a radial structure from the lumen outwards. c Early passaging and differentiation of these neural progenitors with the NOTCH inhibitor DAPT give rise to large numbers of TBR1/β-iii-tubulin + ve putative layer VI cortical neurons