Table 1.
Detail of the primers and cycling parameters
| Sequence (5′-3′) | Product length (pb) | Annealing temperature (°C) |
|---|---|---|
| p14/ARF | ||
| U | ||
| SpUF : TTTTTGGTGTTAAAGGGTGGTGTAGT | 122 | 58° |
| SpUR: CACAAAAACCCTCACTCACAACAA | ||
| M | ||
| SpMF: GTGTTAAAGGGCGGCGTAGC | 132 | 58° |
| SpMR: AAAACCCTCACTCGCGACGA | ||
| P53 | ||
| SPF:CCCAAGCAATGGATGAT | 379 | 56° |
| SPR:TGACAGGAAGCCAAAGG | ||
| Β-actin | ||
| SPF: CCAGGCACCAGGGCGTGATGGTGGGCATGG | 581 | 56° |
| SPR: AGCAGCCGTGGCCATCTCTTGCTCGAAGTC | ||
| P14/ARF | ||
| Sp F: GTGGCCCTCGTGCTGATG | 207 | 58° |
| Sp R: AGCACCACCAGCGTGTCC | ||
| p53 exons | ||
| Exon 5 | ||
| Sp5F: TGTTCACTTGTGCCCTGACT | 266 | 54° |
| Sp5R: CAGCCCTGTCGTCTCTCCAG | ||
| Exon 6 | ||
| Sp6F: GCCTCTGATTCCTCACTGAT | 160 | 53° |
| Sp6R: TTAACCCCTCCTCCCAGAGA | ||
| Exon 7 | ||
| Sp7F: ACTGGCCTCATCTTGGGCCT | 180 | 56° |
| Sp7R: TGTGCAGGGTGGCAAGTGGC | ||
| Exon 8 | ||
| Sp8F: TAAATGGGACAGGTAGGACC | 230 | 54° |
| Sp8R: TCCACCGCTTCTTGTCCTGC | ||