Table 4.
Backbone (N, Cα, C) RMSD and GDT-TS scores comparing structures of NESG target proteins ZR18 and PfR193A generated with the standard NESG CYANA-CNSw protocol, with the same structures refined with rCS-Rosetta following restraint conversion using PDBStat. These ensembles of NMR conformers are compared with the corresponding X-ray crystal structure coordinates.
Number of Residuesa | rCS-Rosetta vs. CNSw RMSD / GDT-TS /GDT-HA | rCS-Rosetta vs. X-ray RMSD / GDT-TS /GDT-HA | CNSw vs. X-rayb RMSD / GDT-TS /GDT-HA | |
---|---|---|---|---|
ZR18 | 91 | 1.18 Å / 0.86 / 0.68 | 0.98 Å / 0.94 / 0.79 | 1.40 Å / 0.82 / 0.62 |
PfR193A | 114 | 0.42 Å / 0.97 / 0.86 | 0.57 Å / 0.99 / 0.93 | 0.64 Å / 0.99 / 0.89 |
Well-defined residues were determined by PDBStat using the FindCore [35] method.
For target ZR18, the PDB_id of the reference X-ray crystal structure is 2ffm. For target PfR193A, the PDB_id of the reference X-ray crystal structure is 2idu.