Table 3.
RT-qPCR data for 5 B-lymphoid tumor cell lines.
| Cellinje | Kappa Cq | Lambda Cq | Kappa noRT Cq | Lambda noRT Cq | mRNA expression | IHC |
|---|---|---|---|---|---|---|
| U698 | 10.3 | 13.8 | 27 | 23.5 | Kappa > Lambda | Kappa |
| DG75 | 10.4 | 15.9 | 25.2 | 24.2 | Kappa > Lambda | Kappa |
| MC116 | 34.8 | 10.4 | Not detected | 23.5 | Lambda only | Lambda |
| RPMI8226 | 12.2 | 14.4 | 25.1 | 22.2 | Kappa > Lambda | Lambda |
| CCRF-SB | 12.3 | 11.2 | 26.7 | 24.4 | Lambda </≈ Kappa | Kappa |
| Neg. control | >33 | ~30 |
Cq: cycle of quantification (log transcript numbers); noRT: samples without reverse transcriptase; IHC: immunohistochemistry.