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GS and HG increase proglucagon mRNA expression. Semiquantitative RT-PCR of proglucagon mRNA expression in GLUTag cells stimulated standard medium (CTR) or high glucose concentration (25 mmol/L) (HG) in presence of different concentrations of glycated serum (400 [GS] and 800 nmol/L [2GS]). (a) Representative agarose gel. (b) Quantification of densitometries of agarose gel bands. Data are represented as mean ± SE (n = 3). **P < 0.01 versus CTR.
