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. Author manuscript; available in PMC: 2014 Dec 3.
Published in final edited form as: Biochemistry. 2013 Nov 19;52(48):8663–8676. doi: 10.1021/bi401192z

Fig. 5.

Fig. 5

Bilin binding to the lyase TeCpcS. (A) Absorbance (solid) and fluorescence emission (dashed) spectra of HT-TeCpcS purified from recombinant E. coli cells containing pTER13-21 and pPcyA. (B) Absorbance (solid) and fluorescence emission (dashed) spectra of purified HT-TeCpcS from cells purified from recombinant E. coli containing pTER13-21and pPebS. (C) Coomassie-stained SDS-polyacrylamide gel of HT-TeCpcS purified from cells containing pTER13-21 alone (lane 1), pTER13-21and pPcyA (lane 2) or pTER13-21and pPebS (lane 3). Molecular mass standards were loaded in lane S. (D) Zn-enhanced bilin fluorescence of the gel (excitation at 532 nm) in panel C.