Table 1.
Summary of plasmids used in this study
| Plasmid Name | Recombinant proteins produceda | Parent vector | Antibioticb | Reference |
|---|---|---|---|---|
|
| ||||
| pApcAB | Synechococcus sp. PCC 7002 HT-ApcA and ApcB | pET100 | Ap | 18 |
| pApcDB | Synechococcus sp. PCC 7002 HT-ApcD and ApcB | pET100 | Ap | 86 |
| pApcF | Synechococcus sp. PCC 7002 HT-ApcF | pET100 | Ap | 86 |
| pApcF/CpcS | Synechococcus sp. PCC 7002 HT-ApcF and Thermosynechococcus elongatus CpcS | pCOLA Duet | Km | This study |
| pPcyA | Synechocystis sp. PCC 6803 HO1 and Synechococcus sp. PCC 7002 HT-PcyA | pACYC Duet | Cm | 13 |
| pCpcSU | Synechococcus sp. PCC 7002 CpcS-I and CpcU | pCOLA Duet | Km | 13 |
| pCpcBA | Synechocystis sp. PCC 6803 HT-CpcB and CpcA | pCDF Duet | Sp | 13 |
| pPebS | Myovirus HO1 and HT-PebS | pACYC Duet | Cm | 87 |
| pPebS2 | Myovirus HO1 and HT-PebS | pCOLA Duet | Km | This study |
| pTeCpcS | Thermosynechococcus elongatus CpcS | pCOLA Duet | Km | This study |
| pTER13-21 | Thermosynechococcus elongatus HT-CpcS | pET21c | Ap | This study |
| pTER13-30 | Thermosynechococcus elongatus HT-CpcS | pET30c | Km | This study |
| pTER13(R151G) | Thermosynechococcus elongatus HT-CpcS (R151G) | pTER13-30 | Km | This study |
| pTER13(S155G) | Thermosynechococcus elongatus HT-CpcS (S155G) | pTER13-30 | Km | This study |
| pTER13(C2S) | Thermosynechococcus elongatus HT-CpcS (C2S) | pTER13-30 | Km | This study |
| pTER13(C169S) | Thermosynechococcus elongatus HT-CpcS (C169S) | pTER13-30 | Km | This study |
| pTER13(C2S/C169S) | Thermosynechococcus elongatus HT-CpcS (C2S/C169S) | pTER13-30 | Km | This study |
| pHy2 | Synechocystis sp. PCC 6803 HO1 and Arabidopsis thaliana HT-Hy2 | pACYC Duet | Cm | 24 |
a
Proteins that would be produced as fusions are indicated as HT; meaning His tagged.
b
Antibiotic resistance used to select for the presence of the plasmid (Ap: ampicillin; Cm: chloramphenicol; Km: kanamycin; Sp: spectinomycin)