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. 2013 Dec 24;53(2):333–343. doi: 10.1021/bi4014769

Figure 2.

Figure 2

Specific activity of Av1 measured by the standard assay at pH 7.8 following incubation at pH 9.5 and 30 °C for the indicated times. The reactions at pH 9.5 were variations of the full turnover conditions with 0.25 mg of Av1: (●) Av1 and Av2 at a CR of 2.5 with the ATP regenerating system, (◆) Av1 and Av2 at a CR of 2.5 with the nonhydrolyzable ATP analogue AMP-PNP, (▲) Av1 with the ATP regenerating system (without Av2), and (■) Av1 and Av2 at a CR of 2.5 without nucleotide. Error bars indicate the standard deviation for duplicate experiments.