Figure 3.

Component ratio and protein concentration dependence of Av1 inactivation monitored by the head space assay at pH 9.5 and 30 °C. (A) Time-dependent ethylene formation at various CRs with the Av1 concentration fixed at 0.25 mg per reaction. Solid lines are nonlinear curve fits to the data points by eq 1. (B) Initial rate (v₀) and inhibition rate (k_i) calculated according to eq 1. Error bars indicate standard errors from the curve fitting. (C) Initial rate (v₀) and inhibition rate (k_i) determined from progress curves at various protein concentrations and at a constant component ratio of 2.5. The data are plotted in terms of the Av1 concentration using an average of two determinations for each data point.