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. 2013 Dec 24;53(2):333–343. doi: 10.1021/bi4014769

Figure 5.

Figure 5

Time course of ethylene production and ATP hydrolysis during inactivation at pH 9.5. (A) Formation of ethylene and ATP hydrolysis (measured as the production of creatine from the creatine kinase-based ATP regenerating system) in the inactivation reaction at pH 9.5 for CRs of 0.3 and 1.0. Assays contained 0.5 mg of Av1. (B) Ethylene production and ATP hydrolysis in specific activity assays at pH 7.8 for samples from the time course of the pH 9.5 inactivation. Values are normalized to the values at time zero. Error bars indicate the standard deviation from duplicate reactions.