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. Author manuscript; available in PMC: 2015 Jan 24.
Published in final edited form as: J Nat Prod. 2013 Dec 23;77(1):92–99. doi: 10.1021/np400727r

Figure 4.

Figure 4

A concentrated solution of enzyme and gallinamide A was incubated for 30 min and then then diluted. (A) The resulting shift in the enzymatic activity is based on the dose response curve. (B) The subsequent rate of the reaction was monitored for 2 h, and comparison of initial reaction rates showed 12% of the activity with preincubation of gallinamide A (○) as compared to the control (●). The reaction rate was constant over the course of the 2 h monitoring period, demonstrating an irreversible mode of inhibition.