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. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: J Am Coll Cardiol. 2013 Aug 28;63(1):21–29. doi: 10.1016/j.jacc.2013.08.694

Fig. 3. EC-specific overexpression of SOCS1 inhibits GA in mice.

Fig. 3

Aortas from non-transgenic littermate WT and VESOCS1 male B6 mice were transplanted to female B6 recipients and the allografts were harvested at 2 weeks or 3 days post-operatively. (A) Histological analysis of 2-week artery grafts by EVG and immunostaining by anti-SMA and anti-CD45 antibodies with DAPI labeling of nuclei. Representative photomicrographs are shown. Arrowheads mark the internal elastic lamina to delineate the intima from media. Scale bar: 50 μm. (B) Morphometric assessment of artery graft intima, media, lumen, and vessel areas and quantification of CD45+ cells infiltrating the intima and media of each artery graft. (C) EC-specific expression of SOCS1 inhibited pro-inflammatory cytokine-induced JAK2 and STAT3 activation in vessel grafts. Cross sections of WT or VESOCS1 grafts at day 3 were co-immunostained with antibodies to phosphorylated (p)-JAK2 or p-STAT3 and the EC marker PECAM-1 with DAPI labeling of nuclei. Representative images are shown. Scale bar: 50 μm. (D) Quantification of the endothelial signaling data in C. Data are mean±SEM from 5–6 mice per group; *P<0.05, **P<0.001, unpaired t-test.