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. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: J Am Coll Cardiol. 2013 Aug 28;63(1):21–29. doi: 10.1016/j.jacc.2013.08.694

Fig. 4. EC-specific expression of SOCS1 inhibits endothelial adhesion molecule induction and corresponding leukocytic infiltration in GA.

Fig. 4

Aortas from non-transgenic littermate WT and VESOCS1 male B6 mice were transplanted to female B6 recipients and the allografts were harvested at 3 days post-operatively. (A) Transcripts for the endothelial adhesion molecules, ICAM-1 and VCAM-1 from WT and VESOCS1 grafts were quantified by qPCR and normalized to HPRT. (B) Induction of endothelial adhesion molecules was also determined by immunostaining with antibodies to ICAM-1 or VCAM-1 and PECAM-1 with DAPI labeling of nuclei. Representative images are shown. Scale bar: 50 μm. (C) Leukocyte infiltration was determined by immunostaining with antibodies to the pan-leukocyte marker, CD45 or the macrophage marker, Mac3 and endothelium marker PECAM-1. Representative images are shown. Scale bar: 50 μm. (D) Quantification of the number of leukocytes in C. Data are mean±SEM from 4–5 mice per group; *P<0.05, **P<0.001, unpaired t-test.