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. Author manuscript; available in PMC: 2014 Feb 23.
Published in final edited form as: Arthritis Rheumatol. 2014 Jan;66(1):107–120. doi: 10.1002/art.38195

Figure 6.

Figure 6

Overexpression of Opg reverses the bone loss phenotype observed in β-catenin cKO mice. A, Serum osteoprotegerin (OPG) production in Col2-Opg–transgenic mice and wild-type (WT) mice (n = 7 per group) was examined by enzyme-linked immunosorbent assay. B–E, Histologic staining of the tibiae of 2-week-old Col2-Opg–transgenic or WT mice (n = 5 per group) was used to assess formation of the secondary ossification center (B) and changes in bone mass (arrows) underneath the growth plate (C). In addition, osteoclast formation (arrows) was assessed by tartrate-resistant acid phosphatase (TRAP) staining in 4-week-old Col2-Opg–transgenic and WT mice (D and E). F–H, Col2a1-Opg–transgenic mice were bred with β-catenin cKO mice, and the tibiae of 3-month-old mice in each group (n = 6 per group) were assessed by micro–computed tomography for bone destruction (arrows) (F) and changes in bone volume (G) and bone mineral density (H). Results are the mean ± SD. * = P < 0.05 versus controls, by Student’s unpaired t-test. See Figure 1 for other definitions.