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Deglycosylation and biotinylation of TRPM5. (A) Treatment with PNGase F demonstrates that unglycosylated WT TRPM5 has a similar molecular weight to the TRPM5 N932Q mutant. (B) Cell surface biotinylation of HEK293 cells transiently transfected with WT or TRPM5 N932Q mutant constructs shows expression of both WT and mutant at the total level and at the cell surface. (C) Treatment with Endo H removes some of the glycosylation in both input and biotinylated fractions.
