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. 2014 Feb 24;5:40. doi: 10.3389/fpls.2014.00040

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Copyright © 2014 Paul, Rinne and van der Schoot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lipid bodies in the perennial shoot apex. (A) Shoot apex of hybrid aspen (Populus tremula × P. tremuloides) after exposure to short photoperiod results in the accumulation of LBs during initiation of dormancy. LBs are visualized by Sudan Black B. Boxed areas (1–3) are detailed on the right; 1 = Tunica, 2 = Corpus, 3 = Rib meristem/Rib zone. (B–E) Electron microscopic images of LBs in shoot meristem of birch (Betula pubescens) during chilling-induced release from dormancy. (B) Immuno-gold-labeling of 1,3-β-glucanase, peripherally associated with a LB. Arrows point to gold particles that label 1,3-β-glucanase (Form Rinne and van der Schoot, 2004). (C) PD in the cell wall of after removal of callosic dormancy sphincter complexes (From Rinne et al., 2001). (D,E) Membranous inclusions, probably desmotubule-attached cortical ER in LB that dock PD. Monolayer membranes of LBs are visualized by Osmium tetroxide and tannic acid. Black arrowheads point to PD (From Rinne and van der Schoot, 2004). Bars, (A) 50 mm; (B–E) 250 nm.