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. 2014 Feb 24;5:40. doi: 10.3389/fpls.2014.00040

FIGURE 3.

FIGURE 3

Lipid body oleosin localizes at the plasma membrane and co-localizes with callose at plasmodesmata. (A–C) Confocal images of Arabidopsis root hairs in GFP::Ole2 lines showing (A) LB marker-protein, oleosin, GFP::Ole2, (B) in connection with lipid after staining with fluorescent dye Nile red. (C) Overlay with bright field shows that GFP::Ole2 co-localizes with LBs, except at the plasmamembrane, where GFP::Ole2 containing bodies are free of lipid. (D–F) Confocal images of leaf cells of Arabidopsis GFP::Ole2 lines showing PD-callose stained with aniline blue (D), and the LB marker-protein GFP::Ole2 (E). (F) Overlay shows that GFP::Ole2 has low expression in leaf cells, but co-localizes with callose at PD. Bars, 10 mm.