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. 2014 Feb 24;5:58. doi: 10.3389/fpls.2014.00058

Figure 6.

Figure 6

Cells attacked by Erysiphe cruciferarum express EGFP-AtCEP1 in the endoplasmic reticulum and accumulate it around haustoria. Localization of EGFP tagged AtCEP1 in Erysiphe cruciferarum attacked cells of Arabidopsis WT cells (atcep1 mutant plants transformed with the functional reporter PCEP1::pre-pro-3xHA-EGFP-AtCEP1-KDEL) upon fungal leaf inoculation 12 dpi. Brick like epidermis cells close to the main vessel are shown; cell outlines are indicated by white dotted lines (A,D). (A) Brightfield image of fungal structures on the leaf. Six bulbous haustoria are visible in the attacked cell (arrows and arrowheads). (B) EGFP-AtCEP1 shows localization in cortical net like structures and around haustoria. (C) Overlay of (A,B). (D–F) Strong accumulation of EGFP-AtCEP1 can be observed around the haustoria. Pictures display a magnified optical section of the area marked by the blue box in the pictures (A–C) in the level of the intracellular haustoria. Pictures are maximum projections of 24 optical sections (A–C) or 12 optical sections (D,E), respectively, with 0.5 μm increments.