Table 1.
IC50 Values of C. thalictroides Methanol Extract and Purified Saponins on Cell Proliferation/Viability after 48 hours and 6 day Treatment (n = 3).a,b
| number | T47D | Hep3B | ||
|---|---|---|---|---|
|
| ||||
| 48 h | 6 d | 48 h | 6 d | |
| 1 (μM) | 13.0 (12.2 – 13.8) | 11.5 (11.2 – 11.8) | 7.5 (7.11 – 7.98) | 6.6 (6.41 – 6.87) |
| 2 (μM) | 12.6 (12.2 – 13.0) | 10.2 (10.1 – 10.4) | 7.0 (6.63 – 7.39) | 3.5 (3.32 – 3.58) |
| 3 (μM) | 30.1 (29.3 – 30.9) | 25.6 (25.0 – 26.2) | 27.8 (22.3 – 34.7) | 9.0 (8.46 – 9.56) |
| 4 (μM) | 9.5 (8.7 – 10.4) | 10.5 (9.8 – 11.3) | 7.4 (6.5 – 8.5) | 8.8 (7.4 – 10.4) |
| C. thalictroides extract (μg/mL) | 55.6 (52.7 – 58.7) | 32.0 (30.9 – 33.2) | 30.8 (29.9 – 31.6) | 24.7 (23.9 – 25.4) |
| emetinec (1 μM) | 60% (+/− 5%)c | 86% (+/− 11%)c | 66% (+/− 10%)c | 93% (+/−6%)c |
a
95% confidence interval values (95% CI) were provided inside the parentheses.
b
The Digitalis saponin, digitonin (4), and protein translation inhibitor, emetine, were used as positive controls.
c
Emetine data were expressed as % inhibition of the untreated control (average +/− SD).