Table 2.
Genotype-Related Differences in Memory. Performance in the Morris water maze probe trials was used to test working spatial memory. A single probe trial (with the platform removed) was employed immediately after completion of training, and 3 probe trials at 6 days post-surgery to assess memory function.
| A. Platform Latency (sec) | Training | Post-surgery Retention (6 d) | New Target Retention (31 d) | ||
|---|---|---|---|---|---|
| Genotype | Sham | Injured | Sham | Injured | |
| Wild-Type | 4.34±0.66 | 13.0 ±3.4§ | 22.6 ±3.5 | 13.1 ±2.7 | 18.3 ±2.8 |
| Ephx2-KO | 5.11±0.65 | 23.0 ±3.9 | 20.4 ±3.8 | 18.5 ±3.0 | 17.1 ±3.4 |
| B. Platform Frequency (#) | Sham | Injured | Sham | Injured | |
| Wild-Type | 8.43±0.45 | 3.53 ±0.38* | 1.76 ±0.40 | 4.82 ±0.84 | 3.94 ±0.80 |
| Ephx2-KO | 6.59±0.47¥ | 2.15 ±0.44 | 2.22 ±0.44 | 3.67 ±0.93 | 3.59 ±0.88 |
| C. Platform Duration (sec) | Sham | Injured | Sham | Injured | |
| Wild-Type | 6.32±0.50 | 1.43 ±0.16* | 0.65 ±0.16 | 1.52 ±0.23 | 1.41 ±0.20 |
| Ephx2-KO | 4.57±0.36¥ | 0.74 ±0.18 | 0.79 ±0.18 | 1.39 ±0.19 | 0.93 ±0.25 |
| D. Time per Visita | Sham | Injured | Sham | Injured | |
| Wild-Type | 0.75±0.06 | 0.40 ±0.02 | 0.38 ±0.02 | 0.31 ±0.03 | 0.36 ±0.03 |
| Ephx2-KO | 0.74±0.07 | 0.34 ±0.02 | 0.34 ±0.02 | 0.42 ±0.04 | 0.26 ±0.04 |
*
p<0.05 vs. all other post-surgery groups;
¥
p<0.02 vs. wild-type;
§
p<0.05 vs. wild-type injured, Tukey HSD.
a
The time per visit was calculated as (platform duration/platform frequency).
Results are mean ± standard error, with n = 20 for training, and n = 10 animals per post-surgery group.