Table 3.

Cerebral Cortex Eicosanoid Levels (37 d postsurgery). Levels in the contralateral cortex, expressed as pg/mg protein, corrected for recovery. Dihydroxyeicosatrienoic acid (DHET) levels, corresponding to the 4 EETs above, were below the limit of quantification in these studies. Assessments of injured tissue, even weeks after TBI, can be confounded by wound necrosis, tissue cavitation and remodelling at the site of injury (at dissection, only cortex that was neither bloody nor patently necrotic was used). Likely due to these issues, eicosanoid levels in the injured cortex had greater variability than on the opposite side of the brain (data not shown), and no genotype- or injury-related differences were detected in ipsilateral cortex.

		(pg/mg protein)
		Ephx2-KO	Wild Type
5,6-EET	Sham	6.07 ±0.98	9.20 ±3.65
	Injured	6.52 ±1.85	7.75 ±2.49
8,9-EET	Sham	5.81 ±0.45*	2.94 ±0.73
	Injured	1.68 ±0.69	1.78 ±0.82
11,12-EET	Sham	1.27 ±0.48	1.45 ±0.15
	Injured	1.57 ±0.36	1.63 ±0.43
14,15-EET	Sham	6.36 ±1.74	3.56 ±1.40
	Injured	6.19 ±1.32	6.97 ±2.65
15-HETE	Sham	39.3 ±8.3	39.5 ±15.0
	Injured	40.2 ±6.0	39.6 ±11.5
12-HETE	Sham	41.6 ±14.1	22.8 ±4.6
	Injured	41.5 ±11.1	48.5 ±10.7
11-HETE	Sham	48.4 ±9.9	45.0 ±17.0
	Injured	45.3 ±7.3	47.0 ±17.4
8-HETE	Sham	3.82 ±0.80	3.07 ±1.03
	Injured	3.34 ±0.68	4.44 ±1.38
5-HETE	Sham	8.82 ±1.75	8.61 ±2.42
	Injured	10.3 ±1.6	9.44 ±2.18

Mean ± standard error, with n = 5 mice per group.

^*p<0.05, vs. all other groups, Tukey HSD.