Table 2.
Expression of UPR genes in optic nerve microarrays from rsh and msd mice compared to previous northern blotting data in spinal cord.
| Function | Gene | Expression Fold-Change Compared to Controls | ||||
|---|---|---|---|---|---|---|
| Microarrays (
FDR < 0.1) P16 Optic Nerve |
Taqman PCR/Northern Blot P16 Spinal Cord |
|||||
| rsh | msd | rsh | msd | |||
| Myelin | Plp1 | 0.44 ± 0.08 | 0.23 ± 0.05 | 0.31 ± 0.03 a | 0.08 ± 0.01 a | |
| Mbp | 0.56 ± 0.08 | 0.39 ± 0.03 | 0.42 ± 0.05 a | 0.17 ± 0.01 a | ||
| Mog | 0.59 ± 0.07 | 0.37 ± 0.03 | 0.35 ± 0.02 a | 0.08 ± 0.01 a | ||
| Cnp1 | 0.67 ± 0.12 | 0.46 ± 0.04 | n.m. | n.m. | ||
| Cldn11 | 0.66 ± 0.13 | 0.35 ± 0.09 | n.m. | n.m. | ||
| Mag | 0.63 ± 0.10 | 0.41 ± 0.03 | n.m. | n.m. | ||
| UPR | Chop | 2.1 ± 0.40 | 1.9 ± 0.40 | 4.2 ± 1.0 a | 3.5 ± 1.0 a | |
| Atf3 | 1.5 ± 0.20 | 1.7 ± 0.30 | 13.8 ± 3.8 a | 7.5 ± 1.5 a | ||
| Atf4 | 1.3 ± 0.20 | 1.3 ± 0.10 | 1.4 ± 0.07 a | 1.5 ± 0.02 a | ||
| Noxa | n.d. | n.d. | 1.79 ± 0.18 a | 1.67 ± 0.41 a | ||
| Trib3 | 3.2 ± 0.40 | 3.0 ± 0.70 | 72 ± 19 a | 58 ± 10 a | ||
| 4eBP1 | 1.8 ± 0.01 | 1.8 ± 0.01 | 2.8 ± 0.4 a | 4.0 ± 0.5 a | ||
| BiP | 1.7 ± 0.37 | 1.7 ± 0.35 | 1.7 ± 0.4 a | 1.7 ± 0.2 a | ||
| Calreticulin | 1.3 ± 0.36 | 1.4 ± 0.46 | n.m. | 1.2 b | ||
a derived from Taqman PCR; b derived from northern blotting; n.m., not measured; n.d., not detected.