Skip to main content
. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: J Med Entomol. 2013 Sep;50(5):1126–1139. doi: 10.1603/me12242

Table 2.

Comparison of PCR assays in laboratory-reared triatomine bugs fed human or rabbit blood

Blood host Days postfeeding Triatoma dimidiata
Rhodnius prolixus
Stage PCRm PCRv Stage PCRm PCRv
Humana 0 N4 + + N5 + +
5 N4 + + N5 + +
10 N4 + + N5 + +
20 N4 + + N5 + +
31 N4 + + Adult – +
40 N4 + + Adult – +
55 N4 – + Adult – +
69 – – – Adult – –
Rabbitb 4 N4 + + N5 + +
9 N4 + + N5 + +
16 N4 + + N5 + +
20 N4 + + N5 + +
25 N4 + + Adult + +
30 N4 + + Adult – –
42 N4 + + Adult – –
60 N5 + + Adult – –
71 N5 + + – – –

PCRm, mammalian a F/R; PCRv, vertebrate BM1/BM2.

All T. dimidiata and R. prolixus were fed as fourth (N4)- and fifth (N5)-instar nymphs, respectively.

One live specimen of each species was tested at each time point.

a

Seven T. dimidiata and 8 R. prolixus nymphs were fed and tested; four R. prolixus specimens molted before testing.

b

Nine T. dimidiata and R. prolixus nymphs were fed and all but one R. prolixus specimen survived to be tested; two T. dimidiata and four R. prolixus specimens molted before testing.