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. Author manuscript; available in PMC: 2015 Jan 17.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Dec 28;443(3):1092–1096. doi: 10.1016/j.bbrc.2013.12.112

Fig 3. IGF-2 induction of CCND1 requires AHR.

Fig 3

MCF-7 cells were reverse transfected (see methods for details) with cRNAi or AHRi prior to treatment with vehicle (Veh) or IGF-2 (100 ng/mL) for 3hr. (A) Total cellular protein was then isolated and subjected to western blot analysis. The blot was then probed with the indicated antibodies. Relative level of CCND1 and AHR protein was expressed as a ratio of AHR/GAPDH and CCND1/GAPDH. A significant (P < .001) increase in AHR and CCND1 by IGF-2 is indicated by (*). A significant (P < .001) decrease in AHR and CCND1 by AHRi is indicated by (**). (B) Q-PCR analyses of relative levels of CCND1 mRNA, expressed normalized to GAPDH. A significant decrease in CCND1 by AHRi is indicated by (P < .01). Data shown are the means ± S.E. of three independent experiments.