Fig. 3. Rg3-mediated BKCa channel current enhancements were not desensitized and were independent of intracellular Ca2+. (A) The representative peak outward current amplitude at +40 mV from a holding potential of -80 mV was measured and plotted against time before and after repeated applications of Rg3 (100 μM) for 30 s. (B) The normalized currents after repeated Rg3 treatment. BKCa channel currents were not desensitized after repeated treatment of Rg3. Data represented the mean ± S.E.M. (n = 6). (C) Oocytes were first treated with BAPTA-AM (10 μM, 3 h). The representative peak outward current amplitude at +40 mV from a holding potential of -80 mV was measured and plotted against time before and after repeated applications of the indicated concentration of Rg3. (D) The normalized currents after treatment of various concentrations of Rg3 in oocytes pre-treated with BAPTA-AM. Data represented the mean ± S.E.M. (n = 6).
