Fig. 5. The effect of rapamycin on P-glycoprotein in Ras-NIH 3T3/ Mdr cells. (A) Cells were transfected for 48 h with pEGFP-LC3 and then incubated with paclitaxel for 24 h at 37℃. GFP-LC3 distribution was analyzed by fluorescence microscopy. The results presented are representative of at least three independent experiments. (B) The expression of P-glycoprotein was assessed by immunoblotting analysis. Whole cell extracts were prepared 24 h post-rapa-mycin treatment (0.5-100 nM). The expression of P-glycoprotein was assessed by immunoblotting. The results presented are representative of at least three independent experiments (C) Beclin-1-transfected or mock cells were pre-treated with rapamycin for 24 h or vehicle and subsequently treated with or without 1 μM paclitaxel for 2 days. Cell growth was measured by the WST assay. The viability of cells treated with vehicle alone was regarded as 100%. Values represent the mean ± SD of quadruplicate determinants from one of three representative experiments.