Fig. 1. Identification and characterization of mMARVELD1. (A) MARVELD1 amino acid sequences in chimpanzee, human, rhesus monkey, mouse, cattle, zebrafish and frog were aligned by ClustalW http://www.ebi.ac.uk/Tools/clustalw2/ with default settings. The conserved amino acid residues are shaded in gray. The extent of conservation is indicated by the brightness of gray and labeled below according to ClustalW definitions: identical residues (*); conservative substitutions (:); semi-conservative substitutions (.). (B) The expression of mMARVELD1 in NIH3T3 cells and extracts of muscle and brain from mouse strain B6 at P0. mMARVELD1 protein was detected by Western blot with anti- MARVELD1 antibody developed by S. Wang et al. GAPDH was used as a loading control. The molecular weight of endogenous MARVELD1 is approximately 19.