Fig. 2. Correlation of CaMKII activity and iNOS expression with pericyte death in diabetic mice retinas. Double immunofluorescent staining for iNOS and ASMA (pericyte marker) was performed, followed by the TUNEL assay with TMR red on the same frozen sections. Increased iNOS and TUNEL-positive cells were observed in diabetic mice retinas (small and large arrows). These signals co-localized with ASMA-positive pericytes (thick arrows). The diabetes-induced increase in iNOS-positive dead pericytes (arrowheads) was suppressed by AIP treatment. GCL, ganglion cell layer; INL, inner nuclear layer; NFL, nerve fiber layer. Scale bar, 12.5 μm.
