Skip to main content
. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Exp Hematol. 2013 Oct 29;42(2):101–113.e5. doi: 10.1016/j.exphem.2013.10.005

Figure 6. Loss of heterozygosity of wild-type Flt3 occurs after transplantation of Flt3/ITD+/NPMc+ AML.

Figure 6

A) Flow cytometry analysis comparing surface expression of cKit (column 1), lineage and surface Flt3 (CD135) (column 2) and Mac1 and Gr1 (column 3) of donor mouse with AML heterozygous for wt-Flt3 and Flt3/ITD with 3 representative recipient mice. B) PCR of donor leukemic mouse BM without LOH of wt-Flt3 compared to 3 recipients. (Top gel: controls on separate gel than donor, gel for recipient #2 is an inverted image). C) Kaplan-meier survival curve comparing mice transplanted with AML cells from a donor mouse without LOH who secondarily lost heterozygousity of wild-type Flt3 (AML with LOH) to recipients who did not lose heterozygousity (AML without LOH).