FIGURE 3.

Ab-dependent phagocytosis. CFSE-labeled Raji cells were cocultured for 4 h with isolated human classical monocytes (A) or nonclassical, intermediate monocytes (B) (E:T ratio, 3:1) in presence of GA101 GE or WT Abs and in the absence or presence of 10 mg/ml Redimune. KPL-4 cells were cocultured for 4 h with M1 (C) or M2c macrophages (D) (E:T ratio, 3:1) in the presence of GE or WT trastuzumab and 10 mg/ml Redimune. The percentage of phagocytized target cells was determined with flow cytometry as described in Materials and Methods. Triplicates of representative experiments (performed using different targets and GE/WT Ab pairs (GA101, trastuzumab) are shown. Statistical analysis, unpaired t test: **p < 0.01.